This textbook for graduate veterinary students introduce the concept, objectives, and definition of One Health and discuss the advancements made and challenges in One Health around endemic and emerging zoonotic diseases. It provides a framework of basic principles of zoonoses, prevention, control, and eradication. It presents diverse group of zoonoses, including viral, bacterial, rickettsia, fungal, and parasitic. It discusses the vital steps on One health in recognizing the critical importance of collaborative, cross-disciplinary approaches for responding to emerging and resurging diseases, and emphasize wildlife health as an essential component of global disease prevention, surveillance, control, and mitigation. Further, the book examines the potential of new emerging zoonotic disease as a tool for bioterrorism. It also explores the applications and advancements of bioinformatics in veterinary research. This textbook is an essential source for students of veterinary sciences, and also for public health professionals, veterinarians, and physicians.