This detailed volume explores the state of research into the obligate intracellular bacterium, Chlamydia, in order to understand its biology and pathogenesis after the advent of genetics and new research tools. The book covers a comprehensive range of topics including infection models, imaging methods, gene expression analysis, genetic manipulation, and assays of the intracellular infection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Chlamydia trachomatis: Methods and Protocols serves as a vital resource for scientists working to study this important bacterial pathogen that is a prevalent cause of human infection and disease. Chapters 13, 22, and 23 are available open access under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License via link.springer.com.
