This volume explores techniques for studying, in detail, cytoskeletal structural and dynamic aspects that regulate cell organization and affect cell-cell interactions. The book features methods involving numerous areas of research, including the production of different relevant cytoskeletal proteins in native conditions, either in cells or in vitro, coupled to CCT chaperonin isolation for correct folding, the isolation of relevant organelles, actin and tubulin polymerization and motor transport, cryoelectron microscopy (CryoEM), post-translational modifications and protein localization, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, The Cytoskeleton: Methods and Protocols serves as an ideal guide for researchers looking to untangle this complex and dynamic network of filaments.
